Journal: Biomolecules

Article Title: Induced Tumor-Suppressing (iTS) Cell-Based Approach for Protecting the Bone from Advanced Prostate Cancer

doi: 10.3390/biom16020240

Figure Lengend Snippet: Inhibitory effects of CM Oy-Lrp5 on TRAMP-C2ras prostate tumor cells. CN = control, CM = conditioned medium, Oy CM = osteocyte-derived CM, and Lrp5 = Lrp5 overexpression. The data are presented as mean ± S.D. (n = 3). The double asterisks indicate p < 0.01. ( a ) Inhibition of MTT-based viability of TRAMP-C2ras prostate tumor cells by CM Oy-Lrp5 . ( b ) Reduction in EdU-based proliferation by CM Oy-Lrp5 . Scale bar: 200 µm. ( c ) Increase in FRET efficiency in the vinculin biosensor by CM Oy-Lrp5 . The observed increase in FRET efficiency implies a decrease in vinculin-mediated molecular force at focal adhesions. Scale bar: 20 µm. ( d ) Inhibition of scratch-based migration of TRAMP-C2ras prostate tumor cells by CM Oy-Lrp5 . Scale bar: 200 µm. ( e ) Inhibition of transwell invasion by CM Oy-Lrp5 . Scale bar: 200 µm. ( f ) Reduction in the growth of 3D tumor spheroids by CM Oy-Lrp5 . Scale bar: 200 µm.

Article Snippet: TRAMP-C2ras prostate tumor cells (ATCC, CRL-2731) were cultured in DMEM/F-12 medium as described [ ].

Techniques: Control, Derivative Assay, Over Expression, Inhibition, Migration

Journal: bioRxiv

Article Title: Salvianolic acids are natural senolytics and increase lifespan in old age

doi: 10.64898/2026.04.29.721790

Figure Lengend Snippet: ( a ) A schematic workflow illustrating the preclinical therapeutic procedure. Two weeks after subcutaneous inoculation and in vivo uptake of PC3/PSC27 recombinants, severe combined immunodeficient (M-NSG) mice were subject to either single agent or combinatorial treatment in a metronomic schedule of several cycles. The illustration was created in BioRender. ( b ) Comparative statistics of tumor end volumes. PC3 cancer cells were inoculated either alone or combined with PSC27 stromal cells before being implanted subcutaneously to the hind flank of M-NSG animals, which were administered with MIT and SAA, either alone or in combination. ( c ) Representative images of in vivo senescence in tumor foci by SA-β-gal staining. Scale bar, 50 μm. ( d ) Comparative statistics of tumor senescence as described in ( c ). ( e ) Transcriptional analysis of a subset of SASP factors expressed in epithelial vs. stromal cells acquired from tumor foci after laser capture microdissection (LCM) to isolate stromal and cancer cells, respectively. Signals were normalized to that of the sample with lowest value in the placebo group. ( f ) Transcriptional analysis of SASP factors and two canonical senescence biomarkers p16 INK4a and p21 CIP1 . ( g ) Statistical assessment of DDR and cellular apoptosis in tumors. Values are shown as the percentage of cells positively stained by IF or IHC specific to γH2AX or cleaved caspase 3 (CCL3), respectively. ( h ) Representative images of IHC staining for CCL3 at the completion of treatment regimens. Scale bar, 50 μm. ( i ) Comparative survival of animals sacrificed upon development of advanced bulky disease. Survival duration was calculated from tissue recombinant injection until death. P values were calculated by a two-sided log-rank (Mantel-Cox) test. DDR, DNA damage response. IF, immunofluorescence. IHC, immunohistochemistry. Data in b , d , e , f and g are shown as mean ± SD and representative of 3 independent biological replicates, with P values calculated by Student’s t -tests. ^, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

Article Snippet: The human prostate epithelial cancer cell line, PC3, was from ATCC and cultured with F-12K media (10% FBS).

Techniques: In Vivo, Staining, Laser Capture Microdissection, Immunohistochemistry, Recombinant, Injection, Immunofluorescence

Journal: Cancer Cell International

Article Title: Discovery and validation of RAB3B as a diagnostic biomarker for prostate cancer with serum PSA below 10 ng/mL based on a multi-omics study

doi: 10.1186/s12935-025-04092-3

Figure Lengend Snippet: Single-cell profiles of prostate cancer and normal prostate tissue. (A) The UMAP plot depicted a comprehensive single-cell atlas, encompassing 91,539 high-quality cells from 4 normal healthy prostate tissues, 13 PSA < 10 ng/mL PCa, and 4 PSA > 10 ng/mL PCa. Various shapes in the bubble chart represented the primary cell types found in the prostate. Outer axis of the circular chart utilized a logarithmic scale to illustrate the total cell count for each cell type. Trajectories were distinguished by four colors (from outer to inner) representing Tissue type, Gleason score grade, BMI range, and pathological stage. (B) Cell types were discerned based on marker genes. (C) Prostate epithelial cells was further classified into 6 different cell types. Volcano plot highlighted the differentially expressed genes (LogFC > 0.5, FDR < 0.05) specific to PSA < 10 ng/mL tumor epithelial cells compared to other cell types (D) as well as other epithelial cells (E) . Venn diagram (F) visually represented the intersection of upregulated genes in PSA < 10ng/mL tumor cells compared to other cell types, and another Venn diagram (G) showed the overlap between upregulated genes in PSA < 10 ng/mL tumor cells compared to other cell types and those detectable in urine

Article Snippet: RWPE-1 and BPH-1 cells were obtained from our institute’s laboratory, and prostate epithelial cancer cells (DU145, LNCaP and C4-2) were purchased from the ATCC cell bank.

Techniques: Cell Characterization, Marker

Journal: Cancer Cell International

Article Title: Discovery and validation of RAB3B as a diagnostic biomarker for prostate cancer with serum PSA below 10 ng/mL based on a multi-omics study

doi: 10.1186/s12935-025-04092-3

Figure Lengend Snippet: Further screening of candidate genes and characterization of their expression profiles. (A-B) The three genes were identified as the optimal feature genes through the LASSO logistic regression algorithm with 10-fold cross-validation. (C-D) Gene filtering by the SVM-RFE algorithm resulted in the identification of five optimal feature genes (maximum accuracy = 0.75, minimum RMSE = 0.25). (E) A Venn diagram illustrated the intersection of the three genes (RAB3B, SLC4A4, and SPON2) obtained from both LASSO and SVM-RFE models. PSA < 10 ng/mL tumors and matched normal tissues were subjected to analysis for the expression of RAB3B (F) , SPON2 (H) , and SLC4A4 (J) . Additionally, pan-cancer expression analysis was conducted for RAB3B (G) , SPON2 (I) , and SLC4A4 (K) . Bubble plots illustrate the expression of RAB3B, SPON2, and SLC4A4 in various cell types (L) as well as different epithelial cells (M) based on single-cell data

Article Snippet: RWPE-1 and BPH-1 cells were obtained from our institute’s laboratory, and prostate epithelial cancer cells (DU145, LNCaP and C4-2) were purchased from the ATCC cell bank.

Techniques: Expressing, Biomarker Discovery

Journal: Cancer Cell International

Article Title: Discovery and validation of RAB3B as a diagnostic biomarker for prostate cancer with serum PSA below 10 ng/mL based on a multi-omics study

doi: 10.1186/s12935-025-04092-3

Figure Lengend Snippet: Function and communication analysis of RAB3B in PCa cells with PSA < 10 ng/mL. (A) GSEA Analysis of RAB3B Function in PSA < 10 ng/mL Tumor Cells. (B) Metabolic Characteristics of Different Types of Prostate Epithelial Cells at the Single-Cell Level: The closer the bubble color is to red, the stronger the correlation. (C) Circular Diagram Showing the Number of Interactions Between RAB3B-EP and RAB3B + EP Cells (as recipients) and Other Cells: The thicker the line, the more interaction pathways. (D) Bubble Plot Displaying Ligand-Receptor Pairs Across All Signaling Pathways: RAB3B + and RAB3B- Tumor Cells as Recipients. The closer the bubble color is to red, the higher the contribution value. Bubble diameter represents probability, with P < 0.05 indicating statistical significance. (E) Circular Diagram Showing the Number of Interactions Between RAB3B-EP and RAB3B + EP Cells (as signal senders) and Other Cells: The thicker the line, the more interaction pathways. (F) Detailed Signaling Pathways for RAB3B + and RAB3B- Tumor Cells as Signal Senders

Article Snippet: RWPE-1 and BPH-1 cells were obtained from our institute’s laboratory, and prostate epithelial cancer cells (DU145, LNCaP and C4-2) were purchased from the ATCC cell bank.

Techniques: Protein-Protein interactions

Journal: Biochemistry and Biophysics Reports

Article Title: Investigating the ABTS-based antioxidant potential and antiproliferative activity of pulp, skin and seeds extracts from Nephelium lappaceum , Manilkara zapota , and Meliccocus oliviformis on human prostate and colon cancer cells

doi: 10.1016/j.bbrep.2025.102257

Figure Lengend Snippet: Effects of the different fruit extracts on the proliferation of the colon cancer cell line HCT116 and the prostate cancer cell line DU145 . DU145 (A) or HCT116 (B) were seeded in a 12-well plate and allowed to grow for 12 h. Then, 500 μg/mLof PBS-resuspended extract derived from the pulp, seeds, and skin of Meliccocus oliviformis , Nephelium lappaceum , and Manilkara zapota were added and cell proliferation was monitored by analyzing the surface area occupied by the cells in each well (% confluence) from photos taken at regular intervals (every 3 h) by the Incucyte live cell imaging system over a period of three days. Data shown are the average ± SD of three independent experiments.

Article Snippet: The HCT116 (colic), and DU145 (prostatic) human cancer cell lines were purchased from ATCC (American Type Culture Collection).

Techniques: Derivative Assay, Live Cell Imaging

Journal: Biochemistry and Biophysics Reports

Article Title: Investigating the ABTS-based antioxidant potential and antiproliferative activity of pulp, skin and seeds extracts from Nephelium lappaceum , Manilkara zapota , and Meliccocus oliviformis on human prostate and colon cancer cells

doi: 10.1016/j.bbrep.2025.102257

Figure Lengend Snippet: Effects of the different fruit extracts on the viability of the colon cancer cell line HCT116 and the prostate cancer cell line DU145 . DU145 (A) or HCT116 (B) were seeded in a 12-well plate and allowed to grow for 12 h. Then, 500 μg/mL of PBS-resuspended extract derived from the pulp, seeds, and skin of Meliccocus oliviformis , Nephelium lappaceum , and Manilkara zapota were added. 24h later, cell viability was assessed using the MTT assay. Data shown are the average ± SD of three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, one-way ANOVA followed by Tukey's multiple comparison test.

Article Snippet: The HCT116 (colic), and DU145 (prostatic) human cancer cell lines were purchased from ATCC (American Type Culture Collection).

Techniques: Derivative Assay, MTT Assay, Comparison